Figure 3
TSE alleviated cartilage degradation on arthritic rats.
(A) Determination of hyaluronidase activity by zymography: (i) Serum hyaluronidase, (ii) Ankle bone joint hyaluronidase. Arrow head indicates the activity bands. M represents the molecular weight markers in kDa. (B) Determination of MMPs activity by zymography: (i) Serum MMPs, (ii) Ankle bone joint MMPs and the gels in this figure are cropped, full length gels are presented in supplementary figure S7. M indicates molecular weight markers in kDa. (C) Protein expression levels of serum MMPs measured by (i) Immunoblotting and their corresponding densitograms of (ii) MMP-13, (ii) MMP-3 and (iii) MMP-9. β-actin, GAPDH and rat serum albumin (RSA) were used as loading controls. Gel in this figure is cropped and the full length gel is presented in supplementary figure S7. Membrane was cut based on the molecular weight, probed with antibody of interest and band of interest were presented. Serum sample are as follows, Lane I – Saline treated, Lane II – Arthritic, Lane III – Arthritic rats treated with Ibuprofen (10 mg/kg), Lane IV – TSE treated arthritic rats (25 mg/kg), Lane V – TSE treated arthritic rats (50 mg/kg) and Lane VI – TSE alone (50 mg/kg). Results are presented as mean ± SEM. a-compared with saline control and b-compared with arthritic group, * p < 0.05.
