Figure 5

Effect of TSE on arthritis induced bone resorption.
The right hind paw ankle bone joint homogenate of control and experimental animals were assessed for the bone resorption and degeneration. (A) Cathepsin K activity as determined by zymography. M indicates molecular weight markers in kDa. Arrow head indicates activity band (~37 kDa). The gel in this figure is cropped, full length gel is presented in supplementary figure S8. (B) Exoglycosidases activity. (C) Cathepsin D activity and (D) Alkaline phosphatase (ALP), acid phosphatase (ACP) and tartrate resistant acid phosphatase (TRAP) activities. Paw ankle joint sample are as follows, Lane I – Saline treated, Lane II – Arthritic, Lane III – Arthritic rats treated with Ibuprofen (10 mg/kg), Lane IV – TSE treated arthritic rats (25 mg/kg), Lane V – TSE treated arthritic rats (50 mg/kg) and Lane VI – TSE alone (50 mg/kg). Results are presented as mean ± SEM. a-compared with saline control and b-compared with arthritic group, * p < 0.05.