Figure 2 | Scientific Reports

Figure 2

From: Total and Proteinase K-Resistant α-Synuclein Levels in Erythrocytes, Determined by their Ability to Bind Phospholipids, Associate with Parkinson’s Disease

Figure 2

The phospholipid ELISA assay specifically detects α-Syn in S-PRBC samples.

(a) Samples of human S-PRBC (0–4 μg protein) were applied to a 96-well microtiter dish containing the indicated phospholipids (Phosphatidylinositol (PI); phosphatidylserine (PS); phosphatidylethanolamine (PE)) at a final amount of 100 μg/well, or without phospholipids (-PL) and processed for the detection of α-Syn using anti human α-Syn antibody, α-Syn #1019). Graph illustrates the means ± SD, n = 3 replicates. (b) Samples of mouse PRBC or S-PRBC from wt or α-Syn-/- (1 μg protein), analyzed by Western blotting using anti- α-Syn antibody, α-Syn #3. Arrows indicate non-specific immunoreactive bands. (c) S-PRBC from wt and α-Syn-/- mice were applied in increasing amounts (0–4 μg protein) into wells of a microtiter dish coated with PI-PS-PE phospholipids (as in (a)), using anti α-Syn antibody, α-Syn#3 or without a primary detecting ab (−1° ab). Graph illustrates the means ± SD of n = 3 repeats.

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