Figure 4 | Scientific Reports

Figure 4

From: Total and Proteinase K-Resistant α-Synuclein Levels in Erythrocytes, Determined by their Ability to Bind Phospholipids, Associate with Parkinson’s Disease

Figure 4

Higher levels of proteinase K-resistant α-Syn in PD samples.

Western blot analysis of PRBC samples (20 μg protein) of healthy controls (a) and PD patients (b), treated with the indicated Proteinase K concentrations for 30 minutes at 37 °C and spun at 20,000 g before loading on a 10% SDS-PAGE. A representative blot, reacted with anti α-Syn (α-Syn#10)19 and anti β-actin abs. Densitometry analysis of Western blots for HC (c) and PD (d). The levels of α-Syn and β-actin are calculated as percent of the signal obtained without proteinase K (set at 100%). Mean ± SD of n = 6 different samples; *, p < 0.01, t-test. Samples of PRBC from healthy (e) and PD (f), analyzed by Phospholipid-ELISA as in Fig. 2a. Graph present mean ± SD of n = 6.

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