Figure 5

α-Syn resistance to proteinase K is acquired in the presence of phospholipids.
Samples of SPRBC (5 μg protein) pre-incubated with PI/PS/PE (1:1:1) or cholesterol/sphingomyelin (1:1), at a final amount of 100 μg, or without any lipids prior to incubation with proteinase K. Proteinase-K resistant α-Syn levels determined by phospholipid-ELISA and presented as percent of total α-Syn, detected without Proteinase K. Mean ± SD of n = 3.