Figure 6 | Scientific Reports

Figure 6

From: Total and Proteinase K-Resistant α-Synuclein Levels in Erythrocytes, Determined by their Ability to Bind Phospholipids, Associate with Parkinson’s Disease

Figure 6

Total and proteinase K-resistant α-Syn are detected in samples of SPRBC by a standard sandwich-ELISA but with a lower efficacy.

Samples of human S-PRBC (0–2.5 μg protein) were applied to a 96-well microtiter dishes and α-Syn levels were determined by phospholipid (PL)-ELISA (as in Fig. 2a) or a sandwich- ELISA, using anti α-Syn ab, Syn-1 (Transduction laboratories) as a capturing antibody. α-Syn detection was obtained with α-Syn #3 antibody (PL-ELISA, a and c) or C-20 antibody (Santa Cruz) (sandwich ELISA, b and d). Graph illustrates the means ± SD (n = 3 replicates) of total α-Syn (filled line) and proteinase K-resistant α-Syn (dashed line) levels of a representative sample in each group.

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