Figure 5
From: In-vivo detection of binary PKA network interactions upon activation of endogenous GPCRs
Benchmarking of PKA reporter dynamics in mouse xenografts.
(A) Time- and dose-dependent effects of isoproterenol treatments on complex formation of RIIb-F[1]:PKAc-F[2] is shown (HEK293 cells stably expressing the Rluc PCA PKA reporter). Luminescent signals from each well were normalized to the last point immediately preceding the administration of isoproterenol. (B) Subcutaneously engrafted HEK293 cells stably expressing the Rluc PCA PKA reporter formed human tumor xenografts in living mice. Shown is time-dependent and non-invasive in vivo luminescence imaging of the PKA reporter in response to application of native coelenterazine (1.2 mg/kg; intra peritoneal, [i.p.]), isoproterenol (2.4 mg/kg mouse; intra venous [i.v.]) and alprenolol (8.0 mg/kg; [i.v.]). The pseudo-color scale indicates intensities of emitted luminescence signals.
