Figure 6
From: In-vivo detection of binary PKA network interactions upon activation of endogenous GPCRs
Benchmarking of PKA reporter dynamics in budding yeast.
(A) Glucose-mediated activations of the S.cerevisiae GPCR Gpr1 leads to cAMP-production and PKA activation. cAMP binds to Bcy1 (R subunit) and triggers dissociation of active Tpk1-3 (PKAc subunits) which phosphorylate substrates and enhance yeast proliferation. (B) Localization of PKA in S.cerevisiae using overexpressed and Venus-PCA fragment-tagged yeast PKA subunits (Bcy1-V[1]:Tpk2-V[2]; V stands for Venus-PCA fragments). (C) Impact of indicated nutrient sugar sources (glucose and galactose) on complex formation of indicated Rluc PCA fused protein pairs (Tpk2-F[1]:aSyn-F[2]; nNos-F[1]:aSyn-F[2]; Tpk2-F[1]:Bcy1-F[2]; SD from 3 independent experiments).
