Figure 7 | Scientific Reports

Figure 7

From: Insights into the Transcriptional Architecture of Behavioral Plasticity in the Honey Bee Apis mellifera

Figure 7

CAGE tags mapping, clustering and TSS identification.

After sequencing, the CAGE tags were mapped to v3.2 of the honey bee Official Gene Set to form clusters (the 3’paired end reads are used to facilitate this mapping). A cluster was identified as a ‘common’ TSS for each group if it had the greatest number of CAGE tags relative to all other clusters and was present in all samples within the group. The location of the forager and nurse TSSs was then compared to determine whether differential TSS selection occurred as a consequence of behavioral state. Additionally, promoter regions identified using CAGE can be scanned for differences in TF binding site occurrence to gain insights into the regulatory architecture controlling each gene’s transcription.

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