Figure 6
From: Pentamethylquercetin (PMQ) reduces thrombus formation by inhibiting platelet function
Effect of PMQ on agonist-induced signal transduction in washed human platelets.
Washed platelets (3 × 108/ml) were stimulated with (A) 0.5 μg/ml collagen, (B) 0.04 U/ml thrombin and (C) 0.3 μM U46619 for 180 s and subsequently lysed with cell lysis buffer. Proteins were separated by SDS-PAGE and transferred to a PVDF membrane. The membrane was probed with the relevant antibodies. Staining of the respective total proteins or GAPDH served as loading controls, all the gels have been run under the same experimental conditions.
