Figure 8

(A) Differential expression profiling of a strong PN- and SN-associated cellulose synthase gene in seven different vegetative (leaf and root) and reproductive (flower bud, ovary, anther, mature pollen and in vitro grown pollen tube) tissues as well as two pod and seed developmental stages of high (ICC 6013) and low (ICC 7346) pod and seed number-containing chickpea accessions using semi-quantitative and quantitative RT-PCR assays. The elongation factor-1 alpha gene was used as the internal control in the RT-PCR. The bars indicate the standard error. *Significance at p ≤ 0.01. (B) Hierarchical cluster display representing the differential expression profile of a strong PN- and SN-associated cellulose synthase gene (validated by GWAS and QTL mapping) determined by comparative global in silico digital transcript profiling. For digital transcript profiling, the whole-genome microarray expression and global transcriptome sequencing data available previously for a similar cellulose synthase gene in different vegetative and reproductive tissues of Arabidopsis, soybean, Medicago and chickpea were used. The colour-scale at the top represents average log signal expression values of genes in various tissues, in which green, black and red signify low, medium and high level of expression, respectively. The tissues and identities of cellulose synthase genes for which the differential expression data available in Glycine max (Gm), Medicago truncatula (Mt), Arabidopsis thaliana (At) and Cicer arietinum (Ca) are mentioned on the top and right-sides of expression map, respectively. We thankfully acknowledge DB and SB for taking this original photograph.