Figure 4 | Scientific Reports

Figure 4

From: Internalization of silver nanoparticles into mouse spermatozoa results in poor fertilization and compromised embryo development

Figure 4

Qualitative analysis of blastocyst stage embryos developed in vitro after IVF with AgNP-treated sperm cells.

(a) A representative blastocyst embryo was stained differentially by OCT4 and CDX2 antibodies 96 h after IVF with AgNP-treated sperm cells. OCT4-positive cells (red) are putative inner cell mass (ICM), whereas CDX2-positive cells (green) are putative trophectoderm. (b) Number of total cells, ICM and TE cells from the blastocyst stage embryos of (a). (c) ICM- and TE-specific mRNA expression analysis in blastocysts developed after IVF with AgNPs-treated sperm cells. The expression levels of ICM and TE associated genes were analyzed by real time RT-qPCR. *p < 0.05, **p < 0.01 and ***p < 0.001 versus the control group (Dunnett’s t-tests).

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