Figure 3
Effect of REST on SRRM3 expression in cells and effect of SRRM3 on REST-003 ncRNA expression and invasiveness in MDA-MB-231 cells.
(A) Expression of different SRRM subfamilies in siRNA-treated MCF-7 and REST-overexpressed MDA-MB-231 cells by our pipeline analysis22,23 of RNA-seq. (B) N-terminal sequences of SRRM3, cfw21 (S. cerevisiae) and SRRM2 (H. sapiens) were compared using the ClustalW2 program. Identical residues in cwf21 domains are in red font for all three SR-related proteins. Stars indicate identical residues in SRRM3 and SRRM2. (C) Effect of si-SRRM3 on REST-003 ncRNA expression in MDA-MB-231 cells using qRT-PCR. (D) (E) Effect of different siRNA treatments on MDA-MB-231 Matrigel invasiveness (D) and REST-003 ncRNA expression (E). Expression levels were normalized to GAPDH, CyclophilinA and/or Actin, converted to MNE and presented as Rel. Exp. after normalization to control cells. Biological replicates are shown on the bar graph as mean plus SEM [paired t-test for (C) and one-way ANOVA with Dunnet test for multiple comparisons for (E)].
