Figure 2
Regulation of de novo lipid synthesis in M. alpina.
Pathways of de novo lipid synthesis are illustrated. Major intermediates and final products are highlighted in black and enzymes found to be regulated at the RNA level during lipid synthesis are indicated by circled numbers. Enzymes involved in glycolysis are highlighted in light blue, pentose phosphate pathway in pink, tricarboxylic acid cycle in yellow, fatty acid synthesis in dark blue and glycerolipid synthesis in orange. Transcriptome analysis was performed in samples from 12, 2 and 0.5 h prior to and 1, 12 and 48 h after nitrogen depletion. RNA expression levels (FPKM in log10 scale) for up-regulated enzymes are plotted in red and down-regulated enzymes in green. PK (EC 2.7.1.40): Pyruvate kinase, PCK (EC 4.1.1.32 and EC 4.1.1.49): Phosphoenolpyruvate carboxykinase, HK (EC 2.7.1.1 and EC 2.7.1.2): Hexokinase, ALDO (EC 4.1.2.13): Fructose 1,6-bisphosphate aldolase, GAPDH (EC 1.2.1.12): Glyceraldehyde 3-phosphate dehydrogenase, ENO (EC 4.2.1.11): 2-phosphoglycerate dehydratase, ACO (EC 4.2.1.3): Aconitase, IDH (EC 1.1.1.42): Isocitrate dehydrogenase, PDH (EC 1.2.4.1): Pyruvate dehydrogenase, OGDH (EC 1.2.4.2): Oxoglutarate dehydrogenase , SUCLG (EC 6.2.1.4): Succinyl-CoA synthetase, MDH (EC 1.1.1.37): Malate dehydrogenase, ACLY (EC 2.3.3.8): ATP-citric lyase, ACS (EC 6.2.1.1): Acetyl-CoA synthetase, ALDH (EC 1.2.1.3): Aldehyde dehydrogenase, ADH (EC 1.1.1.2): Alcohol dehydrogenase, GPAT (EC 2.3.1.15): 3-glycerophosphate acyltransferase, DGAT (EC 2.3.1.20): Diacylglycerol O-acyltransferase, ACSL (EC 6.2.1.3): Acyl-CoA synthetase, CRAT (EC 2.3.1.7): Carnitine O-acetyltransferase, LIP (EC 3.1.1.3): Triacylglycerol lipase, G6PD (EC 1.1.1.49): Glucose-6-phosphate dehydrogenase and PGD (EC 1.1.1.44): Phosphogluconate dehydrogenase.
