Figure 1
From: The p53 binding protein PDCD5 is not rate-limiting in DNA damage induced cell death
Analysis of PDCD5 expression in response to DNA damage.
(a) Hct116, A549 and U2OS cells were treated with 10 μg/ml etoposide or irradiated with 10 Gy. mRNA levels of PDCD5, p21 and BAX were determined by RT-qPCR. Bars represent means of n = 2 experiments performed in duplicates ± SD. (b) Hct116, A549 and U2OS cells were treated as indicated and protein lysates prepared at the indicated times for immunoblotting. (c) Hct116 cells were treated with 20 μg/ml cycloheximide or cycloheximide plus 10 mJ UV irradiation and protein lysates were prepared. IκB degradation was monitored as a control for successful treatment. Western blots shown are representatives of two independent experiments yielding comparable results.
