Figure 6

Normal cell death in response to DNA damage in PDCD5 knockdown cells.

(a) 293T cells were transfected with siRNA targeting PDCD5 and treated as indicated. 24 h after treatment, MTT assay was performed and metabolic activity determined relative to untreated controls. Data represent mean ± SEM; n = 3. (b) Hct116 lines harbouring an inducible shRNA targeting PDCD5 were treated as indicated. After 24 h MTT assay was performed and survival determined relative to untreated cells. Data represent mean ± SEM; n = 3–6. (c) The same Hct116 lines as in (b) were treated as indicated and apoptosis was determined by AnnexinV/PI staining after 24 h. Data represent mean; n = 2–6. (d) The same Hct116 lines as in (b) were treated as indicated and colony formation was determined as specified in the material and methods section. Survival was calculated relative to untreated controls. Data represent mean ± SEM; n ≥ 3 (e) SV40 immortalised wt MEF harbouring two different constitutive shRNAs targeting PDCD5 were treated as indicated. 24 h after treatment, MTT assay was performed and survival determined relative to untreated cells. Data represent mean ± SEM; n = 3; ^* p < 0.05 vs wt.