Figure 8
From: The p53 binding protein PDCD5 is not rate-limiting in DNA damage induced cell death
Knockdown of PDCD5 does not alter cell cycle distribution.
(a) Hct116 cells with inducible PDCD5 shRNA were treated with doxycycline for 5 days followed by γ-irradiation for the indicated times. Cell cycle distribution was assessed by combined PI and α-Histone H3 (Ser10) staining and quantified by flow cytometry. Data represent mean ± SEM; n = 3-4. (b) Representative FACS profiles of Hct116 cells left untreated or exposed to 5 Gy of irradiation and analysed 10 h later. (c) Hct116 cells with inducible PDCD5 shRNA were treated with the indicated DNA damaging agents and cell cycle distribution was determined by combined PI and pH3 staining and FACS analysis. Data represent mean ± SEM, n = 3.
