Figure 6
From: Mannan-modified adenovirus targeting TERT and VEGFR-2: A universal tumour vaccine
IFN-γ production induced by combined vaccine immunization.
The spleens of mice subjected to a complete immunization course were evaluated for IFN-γ production in vivo using a mouse IFN-γ ELISA kit (A). The amount of IFN-γ in the combined vaccine group was much higher than that in controls. Splenocytes were isolated from mice and incubated on a microplate at 37 °C for 4 h. Two concentrations of proteins (0.5 mg/ml VEGFR-2 with 0.5 mg/ml TERT proteins or 0.25 mg/ml VEGFR-2 with 0.25 mg/ml TERT proteins) were added to each well to stimulate splenocytes. The splenocytes isolated from the combined vaccine group higher levels of IFN-γ than the control groups as determined by the ELISPOT assay (B). (p < 0.05, variance test).
