Figure 4
Elevated tissue omega-3 PUFA increases the expression and activity of intestinal alkaline phosphatase (IAP).
(a & b) Relative mRNA expression of two major IAP isozymes (AKP 3 and AKP 6) in the intestine of 20 month old WT (n = 4) and fat-1 mice (n = 5); (c) Western blot analysis showing the protein levels of IAP in WT (n = 5) and fat-1 mice (n = 5); (d) Representative immunofluorescent images showing the difference in IAP expression in of small intestine between WT and fat-1 mice; (e) Total IAP activity and (f) LPS-dephosphorylating activity of small intestinal tissue from WT (n = 4) and fat-1 (n = 5) mice. Data are expressed as mean ± SE. Significance was determined by unpaired two-tailed student T-test. *P < 0.05; ** P < 0.01; *** P < 0.001.
