Figure 5
Fecal IAP from fat-1 mice can transmit to WT mice and alter their gut microbiota, metabolic endotoxemia and inflammation.
Fresh feces collected from the separately housed WT and fat-1 (n = 10/group) and co-housed WT and fat-1 mice (n = 4/group, 2 WT+2 fat-1 in a cage) were separated into bacteria-free stool supernatant (BFSS) and bacterial pellet fractions. The levels of IAP in the whole stool (a), BFSS (b) and bacterial pellets (c) were measured using an IAP assay kit. (d) Schema showing the animal groups and treatments. The BFSS and bacterial pellets from fat-1 mice were transferred by daily gavage or drinking water into WT mice (n = 5) that were simultaneously given a high n-6 diet. Two months after the treatments, the animals were subjected to analysis for changes in LPS production-related bacterial groups in the stool (e–h), markers of endotoxemia (i, j) and inflammation (k) and intestinal permeability (l). Data are expressed as mean ± SE. Data with different superscript letters (a–c) are significantly different (P < 0.05) according to one-way ANOVA with Tukey test. Before and after treatment values (e–l) were analyzed by using paired T-test. *P < 0.05; **P < 0.01.
