Figure 3 | Scientific Reports

Figure 3

From: The EGFR mutation status affects the relative biological effectiveness of carbon-ion beams in non-small cell lung carcinoma cells

Figure 3

Repair of X-ray- or carbon-ion beam-induced DSBs in EGFR-mutant or wild-type NSCLC lines assessed by immunofluorescence staining of γH2AX.

Cells were exposed to X-rays (6 Gy) or carbon-ion beams (2 Gy) in the presence or absence of the DNA-PKcs inhibitor NU7441 (10 μM) and stained with an antibody to γH2AX 24 h post-irradiation. The number of γH2AX foci per nucleus was scored in 30-50 cells for each experimental condition using a fluorescence microscope at ×100 magnification. The results of a representative experiment are shown as box plots. (a) X-rays. (b) Carbon-ion beams (C-ion).

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