Figure 3
Cryopreservation in Reprogramming.
A. Human ESC dissociated before preservation on the plate. H1 hESCs were dissociated on plate before cryopreservation. B. H1 ESC recovery after cryopreservation on plate. Three identical sets of H1 ESCs were prepared on 48 well-plates. One set was directly stained with APS staining, the other two sets were cryopreserved either with or without dissociation and cells were then recovered in E8 and later stained by APS staining kit. C. Cryproservation efficiency on plate. The cells from 3B were counted by flow cytometry, 6 untreated and 6 EDTA-treated samples (p < 0.01) D. iPSC dissociation for cryopreservation. iPSC reprogramming plates were treated with EDTA/PBS before cryopreservation. E. On-plate preservation of iPSC Colonies. Original iPSC reprogramming plates were directly preserved and recovered after brief EDTA dissociation. F. Cryopreservation of expanded iPSC colonies. Two sets of iPSC clones were cryopreserved two days after in-well dissociation.
