Figure 4 | Scientific Reports

Figure 4

From: A cost-effective and efficient reprogramming platform for large-scale production of integration-free human induced pluripotent stem cells in chemically defined culture

Figure 4

Cost-effective and efficient reprogramming platform and characterization.

A. Flow chart for reprogramming of multiple lines. B. Typical flow cytometry profile of iPSCs. iPSCs were expanded for two passages after reprogramming and stained with Tra1–60 and NANOG antibodies with fluorescent markers. C. Average staining from 189 iPSC clones (derived from 72 cell lines). 189 iPSC clones were clonally expanded and stained and the average NANOG/Tra1–60 positive ratios were shown D. and E. Pluripotent and differentiation gene expression profile in qPCR analysis and Fluidigm Biomarker qPCR analysis.

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