Figure 4 | Scientific Reports

Figure 4

From: Production of monoclonal antibodies against GPCR using cell-free synthesized GPCR antigen and biotinylated liposome-based interaction assay

Figure 4

Subtype specificity of anti-DRD1 mAbs.

(a) Cross reactivity against human DRD1 homologs determined by BiLIA. White indicates that the mAb bound to the homolog. (b) DRD1 distribution in mouse brain detected by rabbit mAb obtained in this study. The sagittal plane section was stained by rabbit mAb clone Ra60. Scale bar: 1mm. (c–e) Distribution of dopamine receptors in mouse striatum. Red, rabbit anti-DRD1 mAb clone Ra60; green, guinea pig anti-DRD2 polyclonal36; blue, goat anti-MAP2 polyclonal antibody37. Neurons with Drd2-negative and Drd2-positive perikarya are labeled with D2(–) or D2(+), respectively. Scale bar: 10 μm.

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