Figure 5 | Scientific Reports

Figure 5

From: Production of monoclonal antibodies against GPCR using cell-free synthesized GPCR antigen and biotinylated liposome-based interaction assay

Figure 5

mAbs against three GPCRs.

(a) BiLIA. (b) ELISA. (c) Western blotting. Cell-free synthesized GPCR antigens were subjected to SDS-PAGE and blotted on a PVDF membrane. The blotted membrane was cut into rectangles to separate lanes and each piece was treated with the corresponding mAb, respectively. The immunoreactive signals were visualized by anti-mouse IgG-HRP and ECL reagent. Filled and blank arrowheads indicate monomer and dimer band of GPCR antigen, respectively. (d) Immunoprecipitation. GPCR/liposome complex was incubated with antibody-captured magnetic beads. Immunoprecipitated GPCR was visualized by Western blotting.

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