Figure 1 | Scientific Reports

Figure 1

From: Generation of a variety of stable Influenza A reporter viruses by genetic engineering of the NS gene segment

Figure 1

PTV-1 2A mediated processing of NS1 and NEP does not compromise viral replication.

(A) Schematic representation of the wild type NS segment and the NS1_2A_NEP segment. In the latter mutation of splice donor (SD) and acceptor site (SA) prevents splicing. NS1 and NEP are co-translationally separated by the recoding activity of the 2A peptide of porcine teschovirus 1 (PTV-1). Note that the sizes of the respective genes are not in scale (B) Viral growth of SC35MWT and SC35MNS1_2A_NEP in MDCK-II cells. Viral titers in the supernatant of cells infected at an MOI of 0.001 were determined by plaque assay at the indicated time points post infection. Error bars represent standard error of the mean from three independent experiments. (C) Determination of the expression levels of NS1 and NEP in A549 cells infected with SC35MWT or SC35MNS1_2A_NEP at an MOI of 0.1 at the indicated time points by Western blot analysis. The immunoblot (IB) was carried out with NS1- and NEP-specific antibodies. Detection of tubulin served as loading control. Proteins corresponding to the size of the detected bands are indicated

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