Table 1

From: Generation of a variety of stable Influenza A reporter viruses by genetic engineering of the NS gene segment

SC35M

Positive plaques after 4 passages in culture

after one passage in BALB/c mice

NS1_2A_GFP_2A_NEP

39/39

100/100

NS1_2A_Azurite_2A_NEP

44/44

100/100

NS1_2A_dsRed_2A_NEP

41/41

100/100

NS1_2A_RenLuc_2A_NEP

10/10

n.d.

NS1_2A_GLuc_2A_NEP

10/10

n.d.

PB2_2A_GLuc

10/10

n.d.

PB2_2A_GFP

1/123*

n.d.

PB2mod_2A_GFP

12/12

n.d.

NS1_2A_Cre_2A_NEP

10/10**

10/10

  1. Table 1.  Genetic stability of reporter viruses in cell culture. To determine the stability of the reporter genes encoded by the indicated viruses, A549 cells were infected at an MOI of 0.01. After 4 subsequent serial passages in A549 cells, plaque assay was performed for further analysis. To measure the stability of reporter genes after a passage in mice, plaque assay was performed on lung homogenates 2 days post infection. For fluorescent reporter viruses, plaques were analyzed by fluorescent microscopy. For the luciferase encoding viruses individual plaques were isolated and used to infect MDCK cells to determine luciferase activity. For the Cre-encoding virus, plaques were transferred to Calu-3 cells containing the loxp-dsRed-loxp-eGFP expression cassette in the presence of ribavirin (100 μM). *already after one passage. **passaging performed on MDCK-II cells. n.d.: not determined.
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