Figure 5
The autophagy induced by CTAB-coated GNRs is mediated through a mitochondrial pathway.
(a) HCT116 cells were treated with 0.25–4 nM CTAB-coated GNRs for 24 h and the cells were then subjected to flow cytometry analysis of the mitochondrial membrane potential. (b) ATP relative level detected by a luminometer. The ATP level of untreated cells was set at 100%. (c) Cells were treated with 2 nM CTAB-coated GNRs alone or in combination with 2 mM CsA for 24 h. The expression of LC3 was detected by Western blotting. Cropping lines are used in the figure. Full-length blots are presented in Supplementary Figure 3. The gels have been run under the same experimental conditions. (d) Detection of acidic vesicular organelles with acridine orange staining.
