Figure 7
Cytotoxicity studies of cells treated with GNRs of different aspect ratios.
Cells were treated with GNRs with four different ARs coated with CTAB (CTAB-1, CTAB-2, CTAB-3, CTAB-4) at 2 nM for 24 h, 48 h or 72 h. (a) Cell viability was determined by a trypan blue exclusion assay after 24, 48, or 72 h of treatment with CTAB-coated GNRs with different aspect ratios.Western blot analysis of the cell apoptosis marker PARP, caspase-9 (b) and the autophagy marker LC3 (c). Cropping lines are used in the figure. Full-length blots are presented in Supplementary Figure 5. The gels have been run under the same experimental conditions.Flow cytometry analysis of (d) intracellular ROS and (e) mitochondrial membrane potential.
