Figure 2 | Scientific Reports

Figure 2

From: Quantitative assessment of single-cell whole genome amplification methods for detecting copy number variation using hippocampal neurons

Figure 2

GC content analysis.

(a) The GC-composition of four samples (bulk-cell sample and DNA amplified by three different single-cell amplification methods). The y-axis is the frequency of normalized reads with different GC contents. The total reads of each sample is normalized to 10 million. The average GC content of the rat reference genome is 41.9% (dashed line). The average GC content of MDA, WGA4, MALBAC and unamplified samples are 43.4%, 41.6%, 46.6% and 41.4%, respectively. (b,c) The GC-bias plot. The relative coverage (y-axis) represents the ratio between the coverage of a sample and the coverage predicted by the reference genome. A relative coverage of 1 indicates no bias. A relative coverage above 1 or below 1 indicates higher or lower coverage than that expected, respectively. The results from the bulk-cell sample (red line) are plotted as a benchmark.

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