Figure 7 | Scientific Reports

Figure 7

From: In Silico Prediction and Experimental Confirmation of HA Residues Conferring Enhanced Human Receptor Specificity of H5N1 Influenza A Viruses

Figure 7

In vitro growth kinetics of KAN-1 (Wt-LP) and H5-HA mutant KAN-1 viruses.

a) MDCK-SIAT1, b) QT6, or c) A549 cells were infected at an MOI of 0.001. The infectious virus and HA titers were determined at the indicated time points in MDCK-II cells by focus assay (FFU/ml) and standardized hemagglutination assay (HAU/ml). Mean values of results represent the averages from three independent experiments and are presented with standard deviations (SDs) indicated by error bars. Asterisks (*) indicate a significant difference (p < 0.05) compared to the parental KAN-1 (Wt-LP) virus while (ns) indicates a non-significant difference. Statistical analysis was performed using repeated measures ANOVA, followed by Bonferroni post hoc test.

Back to article page