Figure 2 | Scientific Reports

Figure 2

From: Visualizing the activity of Escherichia coli divergent promoters and probing their dependence on superhelical density using dual-colour fluorescent reporter vector

Figure 2

Functional organization of the fepA/fes genomic region.

Blue arrows show disposition of genes. Black bars show the transcription start points, predicted by the promoter finder PlatProm10. Red arrows indicate positions of starts, registered by biochemical approaches21 while yellow arrows pointed positions revealed by the 5’-end-specific RNA-seq11. Images below exemplify fluorescence of colonies of E.coli K12 MG1655 transformed by reporter plasmid carrying the fragment with regulatory region of fepA and fes integrated between egfp and mCherry. Positions of primers used for PCR amplification of integrated fragment (primers 1 and 4) and qRT-PCR (primers 1–2 and 3–4) are indicated by small gray arrows.

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