Figure 4
Tensile forces remodel matrix at the leading edge of migrating cohorts.
(a) Representative phase contrast image showing cells invading from a mammary epithelial cell cluster. (b) Confocal reflection image depicting the structure of the collagen matrix surrounding the tissue in (a). (c) Merged image of (a) and (b). High magnification images showing the structure of the collagen (d) far from the invading tissue in (a) and (f) adjacent to the invasive front of the tissue in (a). (e) Rose plot showing fibril angles far from the tissue in (a). Histogram represents fiber angles computed at 900 image locations within (d). (g) Rose plot showing fibril angles adjacent to the invasive front of the tissue in (a). Histogram represents fiber angles computed at 900 image locations within (f). Red line indicates the direction of collective invasion. All images are representative of three independent replicates. Scale bars, 50 μm (a–c), 25 μm (d,f).
