Figure 3
From: XI-006 induces potent p53-independent apoptosis in Ewing sarcoma
XI-006 does not induce double-strand break DNA damage at low micro-molar concentrations
(a) Representative images of γH2AX foci formation (immunofluorescence) from TC252 and U20S cells treated with the indicated concentrations of XI-006 for 4 hrs. Cells were stained with DAPI (blue) and γH2AX (green). (b) Percent nuclei positive for γH2AX foci (>5) from cells treated as in (a) (mean ± STDEV from duplicate wells). (c) Western blot analysis of γH2AX protein levels in TC252 cells treated as in (a). β-Actin was used as the loading control. (d) Representative images of neutral comet assays from TC252 and RDES cells treated with low dose XI-006 (0.075, 0.150, 0.300 μM) for 20 hrs. (e) Quantification of tail length from cells treated as in (d), mean ± SE.
