Figure 2 | Scientific Reports

Figure 2

From: MAP3K3 expression in tumor cells and tumor-infiltrating lymphocytes is correlated with favorable patient survival in lung cancer

Figure 2

Silencing MAP3K3 down-regulates multiple proteins.

(A): Four phospho-proteins (p-AKTpan, p-GSK3β, p-P38α and p-CREB) were down-regulated after MAP3K3 knockdown at 72 h in both the H1299 and H838 cell lines as compared to non-target siRNA control as determined using the Human MAPK antibody array (The original image is in Supplementary Figure S7) and (B): Quantitation of pixel densities were evaluated using Image J software. (C): The four proteins were then verified by Western blotting (The Western blots have been run under the same experimental conditions) and (D): proteins were decreased ~50% as compared to NT when analyzed by Image J software.

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