Figure 3 | Scientific Reports

Figure 3

From: EGCG reverses human neutrophil elastase-induced migration in A549 cells by directly binding to HNE and by regulating α1-AT

Figure 3

EGCG regulated the HNE-induced change in the expression of EMT markers.

(A) Confocal images of ZEB-1 in A549 cells exposed to EGCG, sivelestat sodium and neutrophil elastase. A549 cells were grown to 60% confluence and then incubated in 20 μM EGCG, 4 μM sivelestat sodium and/or 10 nM neutrophil elastase for 24 h. The cells were labeled with an anti-human ZEB-1 antibody, a DyLight 488-conjugated secondary antibody (green) and the Hoechst 33342 stain (blue). (B, C) The protein levels of vimentin were clearly down-regulated in response to EGCG treatment with or without HNE. (B, D) The expression of β-catenin was increased after HNE induction but was significantly decreased after treatment with 20 μmol/L EGCG. The gels were electrophoresed using the identical experimental conditions.

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