Figure 7
EGCG attenuates the neutrophil elastase-induced cell migration via the PI3K/Akt pathway.
(A) Western blot for AAT, IRS-1, pAkt (phosphorylated at Thy308 or Ser473), Akt, p-PI3K, PI3K and GAPDH in A549 cells treated with EGCG or sivelestat sodium with or without HNE. (B-F) The statistical data are presented as histograms of the Western blot results for AAT (B), IRS-1 (C), the pAkt (Thy308)/Akt ratio (D), the pAkt (Ser473)/Akt ratio (E) and the pPI3K/PI3K ratio (F) in A549 cells treated with neutrophil elastase, EGCG and sivelestat sodium. The data are presented as the means ± SEM; n = 3. **P < 0.01; ***P < 0.001. The images for IRS-1, AAT and GAPDH were cropped from the same gel. The images for p-PI3K, p-Akt (T308) and GAPDH were cropped from the same gel. The images for PI3K and p-Akt (S473) were cropped from the same gel. The images for Akt and GAPDH were cropped from the same gel. All gels were electrophoresed using the identical experimental conditions.
