Figure 4
From: In situ imaging and proteome profiling indicate andrographolide is a highly promiscuous compound
Targets validation.
a, Silver-stained (SS) PD samples of in situ AP1 (10 μM, 3 h)-labeled A549 cells. The cut band was subjected to LC-MS/MS analysis and results are summarized in b, Note: N, C, M and E represent nucleus, cytoplasm, mitochondrion and Endoplasmic reticulum, respectively. n/a = Not available. c, PD/WB target validation of A549 cells labeled by AP1 (10 μM, 3 h) with or without NEM treatment. d, Graphical summary of CETSA results of A549 cells treated with WT (100 μM, 3 h). WT/p50 interaction in HepG2 cells were used as a positive control. e, Cell imaging of A549 cells labeled by AP1 (10 μM, 3 h) followed by click chemistry with Rh-PEG-N3. IF = immunofluorescence (λex/em = 630/650–750 nm). (Insets) DIC images. Scale bar = 10 μm. f Concentration-dependent in vitro labeling of recombination proteins (~50 ng) by AP1 (1 h), with or without WT (100 μM). Full size WB can be found in Supplementary Fig. S13.
