Figure 1
Fe65 inhibits cell motility in breast cancer cells.
(A), 5 × 104 control or Fe65 stable knockdown MDA-MB-231 cells were plated into trans-well inserts and 3% FBS was used in the medium at the bottom of 24-well plates to induce the migration and invasion. Cells were fixed and photographed 8 h after plating. (B), 5 × 104 control or Fe65 stable knockdown MDA-MB-361 cells were plated into trans-well inserts and 5% FBS was used in the medium at the bottom of 24-well plates to induce the migration and invasion. Cells were fixed and photographed 16 h after plating. Western blots show efficient Fe65 knockdown and β-actin blots are included as loading controls. Quantitative data are shown as bar graphs with each data point representing analyses in duplicate performed in parallel that were repeated three times (n = 6). Values are means ± SD. P-values were calculated with student’s t test (1 tailed distribution).
