Figure 2
The effect of Fe65 on breast cancer cell motility is acetylation-sensitive.
MDA-MB-231 cells were transfected with control or Fe65 siRNA. 40 h post transfections; cells were treated with DMSO, 1 μM SAHA, 2 μM tubastatin A (Tuba A) or 2 μg/ml ACY1215 for 8 h. (A), Western blot analyses were performed to show efficient Fe65 knockdown and β-actin blot was included to show even loading. (B) and (C), Cell migration (B) and invasion (C) assays were performed as in Fig. 1A and photographed 16 h after plating. Quantitative data were presented as bar graphs and data statistics performed as described in Fig. 1.
