Figure 3
The ability of Fe65 to suppress cell motility depends on HDAC6 status.
WT and HDAC6 KO MEFs were transfected with control or Fe65 siRNA for 48 h. (A), Western blots were performed to show the efficient knockdown of Fe65 and β-actin blot was included to show even loading. (B), 4 × 104 cells were plated into trans-well inserts and cells were fixed and photographed 16 h after plating. (C), Quantitative data were presented as bar graphs and data statistics performed as described in Fig. 1.
