Figure 6
Fe65 inhibits invadopodia formation in breast cancer cells.
(A), MDA-MB-231 cells stably expressing control or Fe65 shRNA were plated onto gelatin-coated glass coverslips and stained for cortactin (green), F-actin (phalloidin, red) and DAPI (blue). Representative immunofluorescence images were included to show invadopodia underneath the cells as orange dots in the merged panels. Quantitative data were presented as bar graphs to show the difference between control and Fe65 knockdown cells. Each data point represents the counting of 30 cells. Values are means ± SD. Statistical analyses were performed with student’s t test (1 tailed distribution). (B), A model depicting our current understanding how Fe65 suppresses breast cancer migration and invasion through the recruitment of Tip60 that opposes the activity of HDAC6 on cortactin acetylation.
