Figure 1 | Scientific Reports

Figure 1

From: Amniotic fluid stem cells provide considerable advantages in epidermal regeneration: B7H4 creates a moderate inflammation microenvironment to promote wound repair

Figure 1

Biological characteristics of hAFS cells

(a) mRNA expression of K19, β1-integrin, K14, K8, K18, Oct4, Sox2, c-Myc, Klf-4, Rex-1 and Nanog in keratinocytes, hAFS cell lines hAFS-09 (passages 3 and 5), hAFS-18 (passage 3), hAFS-27 (passages 3 and 10) and hESCs. Cropped gels were used and the gels were run under the same experimental conditions. Full-length images are presented in Supplementary Figure S4. (b) Flow cytometry analysis of hAFS cell surface markers. hAFS cells (passage 5) were analysed after staining with PE-conjugated control isotype IgG (black peaks) or antibodies against the cell surface proteins B7H1, B7H2, B7H3, B7H4, BTLA, CD40, CD80, CD86, CD133, CD45, HLA-DR, HLA-ABC, K19, K14, K10, K8 and K18. (c) After sub-culturing hAFS ten times in succession, 1 × 105 CD117-positive cells isolated from 5 ml of amniotic fluid reached an average of (6.4 ± 2.3) × 109 cells (n = 4) in approximately one month.

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