Figure 4 | Scientific Reports

Figure 4

From: Amniotic fluid stem cells provide considerable advantages in epidermal regeneration: B7H4 creates a moderate inflammation microenvironment to promote wound repair

Figure 4

GFP-positive hAFS cells directly promote and contribute to wound healing in vivo

(a) Representative photographs of the wounds with GFP-positive hAFS cells, fibroblasts or sham cells at day 0, day 7, day 14 and day 21 are shown. (b) Measurement of wounds remaining in the sham, fibroblast and hAFS cell groups in BALB/c mice. Analysis of variance (ANOVA) versus sham or fibroblast groups. The wounds were measured using the UTHSCSA ImageTool; *p < 0.01. (c) Histological analysis of wounds on BALB/c mice in the sham, fibroblast and hAFS groups at days 4 and 14 (H&E stain). At day 4, the wound edge is indicated by the black dashed lines. The infiltrated inflammatory cells (black arrowheads) and the capillary-like structures (red arrowheads) are indicated in the granulation tissue. At day 14, the repaired skin area is indicated by the black dashed lines and newly formed hair follicles (blue arrowheads) are also shown. Abbreviations: M, muscle; F, hair follicle; P, papillae. (d) Flow cytometry of GFP-positive cells in the wound at 7, 14 and 21 days after wounding. (e) Immunofluorescence analysis of the presence of GFP-positive hAFS cells and fibroblasts in the wound at 7, 14 and 21 days. (f) Tissue sections from hAFS cell-treated wounds at day 7 were stained with K14 and K10 antibodies in red. The co-localization of K14 with GFP and K10 with GFP in the merged images appears orange.

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