Figure 4
From: Acrylamide toxic effects on mouse oocyte quality and fertility in vivo
Acrylamide effects on GV oocyte apoptosis.
(A) Acrylamide induced early-stage apoptosis among GV oocytes. In control oocytes, normal oocytes exhibited fluorescence on the zona, whereas oocytes in early apoptosis exhibited fluorescence on the zona and oocyte membrane. The early apoptosis rate in the acrylamide-treated group was significantly increased. Mouse number = 6; n = oocyte number, control: n = 89, low: n = 54, high: n = 61, error bar = SEM (B) Cropped gels were used for our western blot results. Western blot showed that the apoptosis-related protein p-MAPK expression was increased in the acrylamide group. p-MAPK molecular mass is 42/44 kDa and that of α-tubulin is 52 kDa. Relative p-MAPK and α-tubulin staining intensity were assessed by densitometry. *p < 0.05. Bar = 20 μm.
