Figure 2
From: MiR-19a regulates PTEN expression to mediate glycogen synthesis in hepatocytes
MiR-19a mediates activation of the AKT/GSK pathway and synthesis of glycogen in hepatocytes.
(a and g) Levels of miR-19a in mouse NCTC 1469 cells and HEP 1–6 cells transfected with miR-19a mimics or miRNA mimic control. Activation of the AKT/GSK pathway (b and h) and synthesis of glycogen (e and k) in the NCTC 1469 cells and HEP 1–6 cells transfected with miR-19a mimics. (c and i) Expression of miR-19a in the NCTC 1469 cells transfected with miR-19a inhibitor or miRNA inhibitor control. Phosphorylation of the AKT/GSK pathway (d and j) and synthesis of glycogen (f and l) in the NCTC 1469 cells and HEP 1–6 cells transfected with miR-19a inhibitor. Data represent the mean ± S.D. N = 3 independent experiments. *p < 0.05; **p < 0.01byANOVAtest (vs. control). Full-length blots are presented in the supplementary information (supplementary Figure S2).
