Figure 3
From: MiR-19a regulates PTEN expression to mediate glycogen synthesis in hepatocytes
MiR-19a ameliorates IL-6-induced impaired phosphorylation of the AKT/GSK pathway and synthesis of glycogen in hepatocytes.
Activation of the AKT/GSK pathway (a and b) and synthesis of glycogen (e and f) in the NCTC 1469 cells and HEP 1–6 cells treated with 10 ng/ml IL-6 for 24 h followed by transfection with miR-19a mimics for 48 h. AKT/GSK pathway activation (c and d) and glycogenesis (g and h) in the NCTC 1469 cells and HEP 1–6 cells treated with 10 ng/ml IL-6 for 24 h followed by transfection with miR-19a inhibitor for 48 h. Data represent the mean ± S.D. N = 3 independent experiments. *p < 0.05; **p < 0.01by ANOVAtest (vs.IL-6). Full-length blots are presented in the supplementary information (supplementary Figure S3).
