Figure 5
From: MiR-19a regulates PTEN expression to mediate glycogen synthesis in hepatocytes
PTEN participates in miR-19a-mediated glycogenesis in hepatocytes.
Protein levels and mRNA levels (a) of PTEN in the NCTC 1469 cells transfected with siRNA (si-1519) targeting PTEN mRNA or with negative siRNA control. Down-regulation of PTEN rescued the effects of miR-19a inhibitor suppression on the activation of the AKT/GSK pathway (d and e) and improved glycogenesis (b and c) in NCTC 1469 cells and HEP 1–6cells. PTEN did not affect the glycogenesis after treatment with PI3K inhibitor LY294002 (f and g) . The molecular mechanism by which PTEN participates in miR-19a-mediated glycogenesis in hepatocytes (h). Data represent the mean ± S.D. N = 3 independent experiments. *p < 0.05; **p < 0.01by ANOVAtest (vs. control or miR-19a inhibitor or LY294002). Full-length blots are presented in the supplementary information (supplementary Figure S5).
