Figure 2
The effect of Flot-2 overexpression on the morphology of 6-10B cells.
A, The Flot-2 expression level in 5-8F, 6-10B and other NPC cells was detected by semi-quantitative RT-PCR and Western blotting. The expression of Flot-2 in 6-10B was weaker than that in other NPC cells. B, Semi-quantitative RT-PCR and Western blotting were used to detect Flot-2 expression in 6-10B-Flot-2 cells. The 6-10B-Flot-2 cells achieved a comparable Flot-2 expression level to that in 5-8F cells. C, The morphology of 6-10B and 6-10B-Flot-2 cells observed by inverted microscopy (200×). 6-10B-Flot-2 cells had a mesenchymal-like morphology with lamellipodia. D, Cytoskeleton of 6-10B, 6-10B-Flot-2 cells and 5-8F cells were recorded under confocal laser-scanning microscope. 6-10B-Flot-2 cells exhibited a similar microfilament distribution pattern to 5-8F cells, consisting of a high-density distribution of microfilaments on the cell surface, which precedes the formation of conspicuous lamellipodia and membrane ruffles.
