Figure 2 | Scientific Reports

Figure 2

From: Low-molecular-mass secretome profiling identifies HMGA2 and MIF as prognostic biomarkers for oral cavity squamous cell carcinoma

Figure 2

Separation and identification of LMr proteins from the conditioned media of five OSCC cell lines.

(A) Proteins in conditioned media (500 μg) were separated by tricine-SDS-PAGE and visualized using Coomassie Brilliant Blue staining. The portion of each gel lane below 15 kDa was divided into 35 fractions, excised and subjected to in-gel tryptic digestion. The digested peptides from each fraction were analyzed by LC-MS/MS. (B) Proteins (40 μg) from conditioned media (CM) and cell extracts (CE) of the OC3, OEC-M1, SAS, SCC4 and SCC25 cell lines were analyzed by Western blotting using antibodies against α-tubulin and GAPDH.

Back to article page